Tl11

1368 * MI82-17-1-1-1-B

130 d

aMeans followed by the same letter are not significantly different based on the least significant difference test (p = 0.05).

aMeans followed by the same letter are not significantly different based on the least significant difference test (p = 0.05).

accumulation (Table 1). Inbred lines derived from backcrosses included in Group V and those derived from tropical x temperate crosses included in Group II, which were reported in Table 1, were chosen as examples to provide highlights of the results of the screening assay presented in Tables 2 and 3, respectively. Among the lines included in Group V, five S5 lines (L10 - L15) accumulated significantly (p < 0.05) less aflatoxin than did the recurrent parent from the United States, MP420. L11, L12, L14, and L15 did not differ significantly in aflatoxin accumulation from the resistant US inbred check, MI82. Two pairs of S5 lines (L02 and L10, and L05 and L12), which were advanced to the S3 stage of inbreeding from the same single plant, differed significantly (p < 0.05) in aflatoxin accumulation (Table 2). Of the 57 S5 lines evaluated, 23 accumulated significantly (p < 0.05) less aflatoxin than did their respective recurrent parent. Some of these lines also had aflatoxin contamination levels similar to or lower than the resistant inbred check from the United States, MI82.

The S5 lines derived from the Fi crosses also were divided into groups and screened for aflatoxin accumulation with the kernel screening assay. Significant (p < 0.05) differences in aflatoxin production again were detected amongst the lines within each group (Table 1). All of the S5 lines (TL04 to TL011) differed significantly (p < 0.05) in aflatoxin accumulation from the elite tropical parental line, 1368, but not from the resistant inbred check, MI82 (Table 3). We found two pairs of inbred lines (TL04 and TL09 and TL04 and TZ07) that originated from the same single plant at the S3 stage that had contrasting aflatoxin accumulation levels (Table 3). Thirty-two of the 102 S5 lines evaluated had significantly lower aflatoxin levels than the elite tropical inbred parent. About half of these lines did not differ significantly in aflatoxin accumulation from the inbred check, MI82.

Selection of genetically similar inbred lines for proteome analysis

We defined a pair of inbred lines from the same backcross or F1 cross, expected to share at least 88% common genetic background, as genetically similar. Seven pairs of S5 lines derived from backcrosses and ten pairs of lines extracted from F1 crosses had 88-97% genetic identity but differed significantly in aflatoxin accumulation. Proteomic analyses of kernel, embryo and

Table 4. Eigenvectors of the first three principal component axes (PC1, PC2 and PC3) for the various traits of 54 maize inbred lines grown at Saminaka and Ikenne in Nigeria in 2004.

Trait

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