Most mycoherbicide programs are initiated through surveys to discover fungal pathogens exhibiting bioherbicidal potential, followed by a series of biological and ecological assessments to determine the feasibility of mycoherbicide candidates. However, a pragmatic approach of selecting appropriate mycoherbicide candidates is required and should be considered as a continuum amongst several factors that will ultimately influence the field performance of the fungal pathogen (Figure 1). While nutritional and physical factors are vital during fermentation, down-stream processing is equally important for an efficient mass-production system. Selection of appropriate formulation technology is influenced by fermentation processes and should be based on critical limitations such as shelf life and environmental constraints encountered with mycoherbicide development. Formulation ingredients can affect delivery and application of the mycoherbicide agent. If these ingredients result in the inability to deliver the fungal pathogen to the target weed (e.g., high viscosity and ultimate plugging of equipment), effective weed control will not be achieved. Often, mass-production, formulation, and application can be interrelated, therefore, changes in one of
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