Characterization of Laccase

The enzyme contained 510 amino acid residues and was a single polypeptide of a molecular size of 63 kDa. It contained 11% carbohydrate that is consistent with other laccases (Coll et al. 1993). The first 10 amino acids at the N-terminal were A, I, G, P, V, A, B, L, T, and L and differed from the enzyme from P. cinnabarinus PB, also isolated in Australia, only in the B for D in residue 7 (Eggert et al. 1996). It also showed considerable homology to the laccase of P. ostreatus (Youn et al. 1995), C. (Trametes) versicolor (Bourbonnais et al. 1995), and Coriolus hirsutus (Kojima et al. 1990).

A major band and two minor bands were observed at pH 3 and pH 2.85 and 2.65, respectively, on isoelectric focusing (IEF). Each band stained the laccase substrate guaiacol (10 mM). One band only, corresponding to laccase activity, was obtained on electrophoresis (4-20% gradient gel) under nondenaturing conditions. This band, stained with 10 mM guaiacol, corresponded with the co-electrophoresis protein band.

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment