From Genome Sequence to Gene to Mutant to Function

Research in the last five years has been increasingly driven by the availability of whole genome sequences and cDNA libraries from many plants and microorganisms (National Center for Biotechnology Information, http://www.ncbi.nlm. The Arabidopsis genome sequence was released in 2001 (Arabidopsis Genome Initiative 2001) and the draft DNA sequence of two subspecies of rice was released this year (Goff et al. 2002; Yu et al. 2002). Likewise, several fungal genome sequences are now available for model organisms as well as plant pathogens (http://www-genome.;; Friedrich et al. 2001; Turgeon and Yoder 2002). Consortia of scientists have been established to bring together fungal genomic resources for a research community. These include M. grisea ( and Phytophthora (http://www.; Torto et al. 2002).

Comparative analysis of several fungal genome sequences including that of C. heterostrophus, F. graminearum, and Botrytis cinerea, with those of Neurospora crassa and yeast has led to the identification of putative common essential genes, candidate fungal-specific genes, and candidate pathogenicity genes (Turgeon and Yoder 2002). Systematic analysis of these candidate pathogenicity genes through knock out studies is underway. In addition, genes for polyketide synthases and nonribosomal peptide synthetases are being identified and systematically disrupted since many secondary products from fungi are known to have functions as host-specific toxins.

Was this article helpful?

0 0
10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook

Post a comment