Isozyme Techniques For Fungi And Oomycetes

Many methods have been developed for separating enzyme variants and visualizing isozyme variation. The basic approach is to obtain crude extracts of total soluble proteins, separate the proteins by electrophoresis through some type of solid matrix (usually a gel), then visualize the isozymes by use of chemicals that produce a visual reaction in response to specific enzyme activity. General methods of isozyme analysis have been described elsewhere (Selander et al. 1971; Shaw and Prasad 1970; Soltis et al. 1983) and will not be repeated here. However, aspects of isozyme analysis that are specific to fungi will be discussed briefly. Additional information can be found in previous reviews of isozyme analysis and compilations of recipes specifically adapted for fungi (Micales et al. 1986).

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