The traditional and most widely used approach has been to grow fungus with a host plant in a solid growth medium consisting of one or a combination of the solid growth media such as soil, sand, peat, vermiculite, perlite, clay, or various types of composted barks. The mycorrhizal inoculum has not been conveniently mass-produced by traditional sand-based pot culture techniques and different micro-organisms frequently contaminate it. In addition, the volume and weight of the inoculum produced by solid growth cultures was sometimes too large and bulky to carry and utilize (Wang and Tschen 1994). Alternative particle size distributions of substrates vary the inoculum production, the ideal substrate proposed for optimum production is proposed to be low in nutrients and carbon (Gaur and Adholeya 2000).
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