Properties

The ultra-violet visible (UV/Vis) absorption spectrum of laccase was similar to that observed of other fungal laccases (Coll et al. 1993; Eggert et al. 1996) with a Type I band at 614 nm, corresponding to the blue copper of laccases (Reinhammer and Malstrom 1981) and a broad band at 330 nm indicative of a Type III binuclear copper center (Malkin et al. 1969; Reinhammer 1984). The EPR spectrum of purified laccase had two superimposed signals of Type I and Type II copper centers.

The laccase was stable at 60°C for1 h and still retained much activity after 2 h incubation at 80°C. It differed from the laccase of P. cinnabarinus PB (Eggert et al. 1996) which was inactivated after incubation at 80°C for 1 h. The enzyme was still active in bioreactors run at 37°C for 25 days but lost activity on prolonged treatment at the higher temperatures.

The pH optimum for syringaldazine was between pH 4.4 and 5 and for guaiacol between pH 4 and 4.5. The Km was

0.03 mM for syringaldazine and 0.33 mM guaiacol. Activity was irreversibly inhibited by 0.1 mM sodium azide and by ascorbic acid at 1 mM.

Was this article helpful?

0 0
10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment