The fermentation was conducted in a 2001 aerated polythene drum, packed with 2 cm3 nylon (Scotchbrite®) cubes (Lonergan et al. 1995 b). A reactor cycle of 25 days was used in this instance with the medium continually recycled at 36 ± 1°C. P. cinnabarinus rapidly colonized the packing. Veratryl alcohol (4 mM) was used as a laccase inducing agent. The RBBR was added (500 mg/l) to the medium on day 3 after inoculation. Daily samples were analyzed for glucose, nitrogen, and phenoloxidase (laccase) activity. A sample was also taken 30 min after addition of the dye and is termed D3 + 30.
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