Furnish a description of the genetic and resultant phenotypic modifications of the GMO This should include the origin of the inserted DNA the procedure used to induce the genetic modification and the extent to which it has been characterized

The sunflower was transformed by agro-infection using a construct that contains an antibiotic marker (resistance to hygromycin), an herbicide tolerance gene (the bar gene giving tolerance to Basta), and two resveritol genes from grapevines, giving tolerance to fungal infection. The antibiotic marker gene and the herbicide tolerance gene both come from soil organisms. A single, stable insertion has occurred in one of the plant chromosomes.

Map of the disarmed Agrobacterium Ti-plasmid used to transform sunflower

LB-RB = T-DNA transferred to sunflower

Hm = hygromycin resistance gene with nos promoter and terminator rsvl; rsv2

LB-RB = T-DNA transferred to sunflower

Hm = hygromycin resistance gene with nos promoter and terminator bar = phoshinothricin tolerence gene with 35S promoter and nos terminator

Vir region

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