The aeration intensity must be sufficient to maintain oxygen levels above 2 mg/L, preferably 5 mg/L. The optimal aeration rate is a function of tank size and the density of cysts incubated. Aeration is usually provided by a simple tube connected to the air supply. The tube outlet must be positioned at the lowest point of the tank; if not, nauplii will actively accumulate under the aeration and die of oxygen depletion. In large tanks and high cyst densities, additional oxygenation may be necessary using airstones. Airstones, however, have the inconveniency of producing foam in which newly hatched nauplii tend to be caught. Excessive foaming can be reduced by disinfection of the cysts prior to hatching incubation and/or by addition of a few drops of a non-toxic antifoam agent (e.g. silicone antifoam).

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